Composite

Part:BBa_K5000001:Design

Designed by: jian mo   Group: iGEM23_Squirrel-Guangzhou   (2023-10-11)


introduce new pathway to produce isoprenoid in ecoli


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3473
    Illegal SpeI site found at 3467
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3473
    Illegal SpeI site found at 3467
    Illegal NotI site found at 3498
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3473
    Illegal BamHI site found at 1904
    Illegal XhoI site found at 3507
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3473
    Illegal SpeI site found at 3467
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3473
    Illegal SpeI site found at 3467
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

the three genes have independent RBS and the same T7 promoter


Source

Scck is from S. cerevisiae (AAA34499.1), ATipk is from Arabidopsis thaliana (AAN12957.1),idi is from Ecoli, all of them are codon optimized.

References